normal colon epithelial cell line ncm460  (ATCC)

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: The E3 Ubiquitin Ligase ARIH1 Facilitates Colorectal Cancer Progression by Promoting Oxidative Phosphorylation via the Mitochondrial Translocation of K63-Linked Ubiquitinated PHB1.

doi: 10.1002/advs.202501017

Figure Lengend Snippet: Figure 1. ARIH1 was highly-expressed in CRC tissues and cell lines and correlated with poor prognosis. A) The ARIH1 mRNA expression levels were quantified by qRT-PCR in 30 CRC tissues and matched adjacent normal tissues. B-E) The protein level of ARIH1 detected by IHC in the TMA of 80 CRC patients’ samples. B) Comprehensive tissue microarray schema of ARIH1 expression, C) representative images of ARIH1 expression, D) the relative statistical analysis of H-score, and E) the Kaplan-Meier survival analysis of patients with high (n = 35) and low (n = 45) ARIH1 expression. F) The protein level of ARIH1 was detected by WB in 12 paired CRC tumor and adjacent tissues. G) The mRNA and protein levels of ARIH1 in CRC cell lines and NCM460 cells, evaluated by qRT-PCR and WB respectively. Data was shown as mean±SD of three independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Cell Culture: The human normal colonic epithelial cell line NCM460, HEK293T, along with CRC cell lines, including HCT116, DLD-1, SW620, SW480, LOVO, and RKO, were purchased from Servicebio Technology (Wuhan, China).

Techniques: Expressing, Quantitative RT-PCR, Microarray

Journal: BMC Cancer

Article Title: A novel machine learning-based immune prognostic signature for improving clinical outcomes and guiding therapy in colorectal cancer: an integrated bioinformatics and experimental study

doi: 10.1186/s12885-025-13437-0

Figure Lengend Snippet: Functional analysis of the representative gene APCDD1 in IRPS ( A ) Comparison of the APCDD1 expression level between CRC tissues and normal control tissues based on the GEPIA2 database; ( B ) The expression level of APCDD1 in CRC lines SW620, HT29 and SW480 was detected by qRT-PCR with the normal colon epithelial cell line NCM460 as a control; ( C ) The siRNA interference efficiency of APCDD1 in HT29 cell line was detected by qRT-PCR; ( D ) CCK-8 assays were used to detect the effect of APCDD1 knockdown on HT29 cell proliferation; ( E and F ) Transwell assays were used to detect the effect of APCDD1 knockdown on HT29 cell migration ( E ) and invasion ( F ); ( G ) Apoptosis assays were used to detect the effect of APCDD1 knockdown on apoptosis of HT29 cells. * P < 0.05; ** P < 0.01; *** P < 0.001

Article Snippet: The human CRC cell lines HT29, SW480 and SW620, as well as the human normal colon mucosal epithelial cell line NCM460, were purchased from KeyGEN BioTECH Corp., Ltd (China).

Techniques: Functional Assay, Comparison, Expressing, Control, Quantitative RT-PCR, CCK-8 Assay, Knockdown, Migration